The Queen is an alluring and cryptic beast who captivates men with her charm and devours them in her palace. Very determine to secure her meal, she takes form in any matter of water, whether it be droplets or steam. After kidnapping the infatuated victim from their home, she takes them back to her castle where she performs a ritualistic dance for them. She endows them with a necklace of shells, then she transforms into her monstrous true form and strips them clean of their flesh and muscle, leaving only a smiling skeleton behind. Her strange ocean world can be accessed from seemingly any body of water. She can also take the form of a canine to seduce male dogs.
A thundering storm passed by the farmhouse, leaving behind a bubbling black pool. Courage discovered this puddle, and was frightened to see the reflection of a woman stare back and hiss at him. He tried to tell Muriel and Eustace, but to no avail. Soon after, the farmer began to hallucinate. Images of the mysterious queen appeared to him whenever he entered close proximity to water, and each time he was left dazed and infatuated. Muriel became worried for her husband, who became increasingly disturbed as the visions progressed. That night, the queen emerged from her puddle and entered the farmhouse. She took the sleeping Eustace from his bed and dragged him down below to her home. The next morning, Courage found Muriel crying at the loss of her husband, so he reluctantly dove into the depths of the shrinking puddle. As Courage beheld the Queen's bizarre ritual she performed for Eustace, he saw the necklace of shells she placed on him similar to the ones on the skeleton outside her castle, Courage realizes that she intends to eat him. The dog successfully rescues the farmer, returning to the surface via Muriel's tear puddle. The Black Queen is left behind in her skeleton-moated kingdom after Courage seals the tear puddle with a blow dryer.
Some time later, the Black Queen returned along with many other antagonists for Eustace's dodgeball revenge game. Compulsively hungry, she took one of Muriel's finger sandwiches prematurely, revealing their cover as a "men's club." Later in the game, she appeared using a puddle of Sports Swill that Courage spit up, and assaults him with various sea life. As the end seems near, and the Queen has equipped a rocket conch shell, Courage unleashes a massive scream, causing a tremor that the villains fall into.
- Queen of the Black Puddle
- Ball of Revenge